You probably would get better results with a supersaturated solution of the dye in 60% isopropanol with added dextrin. For details see https://www.urmc.rochester.edu/urmc-labs/pathology/StainsManual/. Click on Table of Contents and follow the links to fined the method. Be sure to use a batch of Sudan black B certified by the Biological Stain Commission. John Kiernan London, Canada = = = ________________________________ From: Gudrun Lang via Histonet <[email protected]> Sent: March 28, 2023 10:30 AM To: [email protected] <[email protected]> Subject: [Histonet] Sudanblack B on FFPET
Hallo! Has anyone experience with Sudanblack B on paraffin slides for staining lipofuszin? A doctor wants the demonstration of the lipoid content of foamy cells or granulocytes in lung. I've found protocols that have incubation-times from 10 minutes to over-night. I've made a saturated Sudanblack B-solution in 70% ethanol and tried it with 10 min on liver without real success. I would appreciate any input and help. Thanks in advance Gudrun Lang Austria _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
