Ay perdón, no conocía que ya existe el medio de montaje Q Path® Safemount 00647520 compatible con el sustituto. Perfecto.
Atentamente, Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Jun 15, 2023 at 12:07 PM Jay Lundgren <jaylundg...@gmail.com> wrote: > I've used all of them and they work fine. There's two flavors, one that > smells like bug spray (aliphatic hydrocarbons based) and the citrus kind. > If you have one of those people in your lab that freaks out about > fragrances (which is an actual, recognized psychosis, btw, it's called > hyperosmia and it can be a symptom of major psychological affective > disorders), they won't be able to enter the building if you switch to a > citrus based product. Your blocks will still smell like oranges after > being cut and filed. > > But anyway, both varieties work fine. I saw the salesman for a citrus > based clearing agent drink some of it to demonstrate its non-toxicity once. > > Neither type of xylene substitute is half as aggressive as xylene in > deparaffinization or clearing, so you'll have to (roughly) double your > deparaffinization and clearing times on the processor and stain line. > You're going to have to write and verify all new protocols for processing > and staining. > > The hardest part is going to be convincing your Medical Director to > change. Pathologists can be some of the most hidebound, unwilling to > change dinosaurs around. > > As far as I know, you still have to keep some xylene in your lab, > unfortunately, because all the coverslipping media are either toluene or > xylene based. Especially if you coverslip using an automated instrument, > as most of the manufacturers specify a particular coverslipping media. So > you still have to keep a fume hood/ventilation over (or even better, under) > your coverslipping area. Unless you wanted to coverslip with a water > soluble media, which is a pain, and not suitable for archival storage. > Maybe you can dilute xylene/toluene based media with xylene substitutes? I > don't know, I've never tried. You might be able to get away with it, but > it would invalidate your coverslipper warranty, if you had one. There's > still going to be xylene/toluene off-gassing from your drying slides. > Someone tell me if they know of a non-xylene/toluene based coverslipping > media, besides water based ones, of course. > > You didn't ask, but the formalin substitutes work fine also. They take > about twice as long as formalin to fix a given specimen and are less > forgiving with thick sections, fatty sections, etc. But they do work, and > yield beautiful nuclear detail and prettier IHCs than 10% NBF. Again, good > luck getting your pathologists to switch over. > > > The health and safety benefits of getting rid of xylene are obvious. > There might also be a financial argument for switching to a xylene > substitute, as the cost of disposing of formalin and xylene in some locales > is becoming prohibitive. > > Sincerely, > > Jay A. Lundgren, M.S. HTL (ASCP) > > On Thu, Jun 15, 2023 at 1:53 AM Monica Aguilera via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > >> Hi! >> >> Does anyone have experience with xylene substitutes like this? >> >> >> https://es.vwr.com/store/product/16702338/vwr-q-path-safesolv-solvent-substitute >> >> Thanks! >> >> Mònica >> >> -- >> Mònica Aguilera Pujabet, DVM, PhD >> Senior Research Officer >> >> Histopathology Facility >> Institute for Research in Biomedicine - IRB Barcelona >> Baldiri Reixac, 10 >> E-08028 Barcelona - Spain >> >> Tel: +34 934033776 <%2B34%20934020546> >> monica.aguil...@irbbarcelona.org <neus.pr...@irbbarcelona.org> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
