Hi Gudrun, This should be useful ( I will send a copy separately)
Henwood, A. F. (2023). Validation of nominally expired antibodies for immunohistochemistry. Biotechnic & Histochemistry, 98(2), 86-93. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children’s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: Gudrun Lang via Histonet<mailto:histonet@lists.utsouthwestern.edu> Sent: Sunday, 2 July 2023 4:59 AM To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> Subject: [Histonet] shelf life of working antibody solutions in IHC Hi all! I have tried to find a general instruction for the shelf life of antibody working solutions. With automated IHC you usually fill the container with the working solution and depending on the frequency of usage they stay on the instrument at roomtemperature (or higher) or are put in the fridge again. The working solutions are up to 10 ml and may last for months. The antibody-diluent is from the same company of the instrument. The titers are in a range from 1:10 to 1:3000. - so a very heterogen situation. How do you handle this? Have you a general rule, when the solution has to be discarded? Is it just a matter of positiv-controls? Thanks in advance Gudrun Lang Biomedical scientist Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
