Hello gang Jores has several references found in a Google search, including a post right here on the Histonet from 2002. It contains Chloral Hydrate which is very difficult to find. Do a search and have a look. Several suggestions.
AnnieinArabia (now retired in Africa) Sent from my iPhone > On 21 Sep 2023, at 7:07 PM, [email protected] wrote: > > Send Histonet mailing list submissions to > [email protected] > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > [email protected] > > You can reach the person managing the list at > [email protected] > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Detergent in heating antigen retrieval (Alonso Mart?nez Canabal) > 2. Re: Histonet Digest, Vol 237, Issue 4 (Eddie Martin) > 3. Re: Long term museum specimen storage (John Kiernan) > 4. Re: Detergent in heating antigen retrieval (Gudrun Lang) > 5. p17 mice brain sections (Alonso Mart?nez Canabal) (Amos Brooks) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 20 Sep 2023 13:24:55 -0600 > From: Alonso Mart?nez Canabal <[email protected]> > To: Histonet <[email protected]> > Subject: [Histonet] Detergent in heating antigen retrieval > Message-ID: > <cag12gaftso3j4bhvdzf+oxz7p_td64t3hfw8+buinjodjzr...@mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Dear histoneters, > I have performed heating antigen retrieval with citrate buffer > pH 6 with 0.05% tween-20, however I have seem recipes with no detergent, > anyone has any experience or knowledge if it is better with or without the > detergent? > Thank you! > > -- > Dr. Alonso Mart?nez Canabal PhD > Profesor Asociado "C" > Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM > Investigador Nacional "I" > 56224833 > > > ------------------------------ > > Message: 2 > Date: Wed, 20 Sep 2023 21:24:14 -0400 > From: Eddie Martin <[email protected]> > To: [email protected] > Subject: Re: [Histonet] Histonet Digest, Vol 237, Issue 4 > Message-ID: > <cadpzgvqw9mmazyhvzn+j0w+x7docwhxoug_epvkwje_qhyr...@mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Thought on alternative for Sudan Black. I don't use this stain...an > alternative is an Oil Red O stain. Oil Red O is done on frozen > sections...but you can also deparaffinize FFPE sections to water, and then > perform your Oil Red O stain. > > I hope this helps. > > Very Respectfully, > Eddie Martin > > Eddie Martin, HTL, QIHC > The National Institutes of Health > Bone Marrow Service > 10 Center Drive > Building 10, Room 2C360 > Bethesda, MD 20892 > Office: 301-594-2054 > > >> On Thu, Aug 10, 2023 at 12:59?PM <[email protected]> >> wrote: >> >> Send Histonet mailing list submissions to >> [email protected] >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> [email protected] >> >> You can reach the person managing the list at >> [email protected] >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> Today's Topics: >> >> 1. Happy Hump Day Histopeeps!! Summer is Almost OVER!!! Here are >> some exciting new opportunities for you and your friends! >> ([email protected]) >> 2. Sudan Black B (Betsy Molinari) >> >> >> >> ---------- Forwarded message ---------- >> From: <[email protected]> >> To: <[email protected]> >> Cc: >> Bcc: >> Date: Wed, 9 Aug 2023 14:13:39 -0400 >> Subject: [Histonet] Happy Hump Day Histopeeps!! Summer is Almost OVER!!! >> Here are some exciting new opportunities for you and your friends! >> Happy Hump Day Histopeeps!! >> >> It is hard to believe the summer is almost over. The kids are going back >> to school and Labor Day is just a few weeks away! >> >> A few more weeks after that ?Halloween; Then Thanksgiving, Christmas, and >> New Year?s Day!! >> >> ? Looking for a new job in the Fall? >> >> ? Planning a job change after the holidays? >> >> ? Considering making a move in 2024? >> >> Let?s Get The Ball Rolling!!! >> >> Histonetters! >> >> **Are you a histo tech looking for something better? >> >> **Are you a new or recent graduate of a histology school? >> >> **Are you a traveler transitioning into a permanent position? >> >> **Are you a Histotech looking to advance into leadership? >> >> **Are you a Supervisor looking to advance to lab management? >> >> Let?s Get The Ball Rolling!!! >> >> All you have to do is contact me at [email protected] >> <mailto:[email protected]> or call/text me at 407-353-5070 or call me >> at >> 866-607-3542 my office is open M-F 7am-8pm EST or by appointment. >> >> >> >> If you know of anyone else who might be interested in subscribing to >> RELIA?s Histology Careers Bulletin, please feel free to pass this along to >> them. >> >> I have exciting opportunities in: >> >> ? Leadership >> >> ? Tech Support >> >> ? Mohs >> >> ? GI >> >> ? Derm >> >> ? Clinical >> >> ? AP >> >> ? Research >> >> These Opportunities are located in: >> >> ? Florida ? Brand New Lab! >> >> ? Washington >> >> ? South Carolina >> >> ? Arizona >> >> ? California >> >> ? Massachusetts >> >> ? Wisconsin >> >> ? Tennessee >> >> ? Georgia >> >> ? Alabama >> >> I invite you to join my group on Facebook: >> >> www.facebook.com/groups/histotechnologists >> <http://www.facebook.com/groups/histotechnologists> >> >> Have a Great Week! >> >> >> >> >> >> Thanks-Pam >> >> Right Time, Right Place, Right Move with RELIA! >> >> Providing excellent service exclusively to the Histology Community! >> >> Thank You! >> >> Pam M. Barker >> >> Pam Barker >> >> President/Senior Recruiting Specialist-Histology >> >> RELIA Solutions >> >> Specialists in Allied Healthcare Recruiting >> >> 5717 Red Bug Lake Road #330 >> >> Winter Springs, FL 32708-4969 >> >> Phone: (407)657-2027 >> >> Cell: (407)353-5070 >> >> FAX: (407)678-2788 >> >> Toll free: (866)60RELIA or (866)607-3542 >> >> E-mail: [email protected] <mailto:[email protected]> >> >> https://www.facebook.com/RELIASolutionsforhistologyprofessionals >> >> www.linkedin.com/in/reliasolutions >> <http://www.linkedin.com/in/reliasolutions> >> >> I invite you to join my group on Facebook: >> >> www.facebook.com/groups/histotechnologists >> <http://www.facebook.com/groups/histotechnologists> >> >> Follow my hashtags to make your day great and your career greater! >> >> #ilovemyhistopeeps >> >> #jobs4myhistopeeps >> >> #histologyiscool >> >> #histologyjobs >> >> #histologycareers >> >> #histology >> >> >> >> >> >> >> >> >> >> >> >> ---------- Forwarded message ---------- >> From: Betsy Molinari <[email protected]> >> To: Histonet <[email protected]> >> Cc: >> Bcc: >> Date: Thu, 10 Aug 2023 14:57:31 +0000 >> Subject: [Histonet] Sudan Black B >> Hi, >> I have been asked to do a Sudan stain on a heart biopsy for lipofuscin. >> The biopsy is in a paraffin block. They are looking to better report and >> understand the IHC. I am totally unfamiliar with this stain. I did some >> reading but have been unable to find a protocol for paraffin sections. I >> found a reference to Sheehan & Hrapchak (1973) but unfortunately I don't >> have that edition. Any ideas would be greatly appreciated . >> >> Betsy Molinari HT (ASCP) >> Texas Heart Institute >> Cardiovascular Pathology >> 1101 Bates St. >> Houston, Texas 77030 >> 832-355-6524 >> >> Betsy Molinari, HT (ASCP) >> Sr. Histology Research Technician >> CV Pathology Research >> >> The Texas Heart Institute (r) >> 6770 Bertner Avenue, MC 1-283 >> Houston, TX 77030 >> >> Office: 832-355-6524 | Fax: 832-355-6812 >> Email: [email protected] >> texasheart.org<https://www.texasheart.org/> | texasheartmedical.org< >> https://www.texasheartmedical.org/> | facebook< >> https://www.facebook.com/Texas.Heart.Institute> | twitter< >> https://twitter.com/Texas_Heart> >> >> CONFIDENTIALITY NOTICE: This email and attachments contain information >> that may be confidential or privileged. If you are not the intended >> recipient, notify the sender at once and delete this message completely >> from your information system. Further use, disclosure, or copying of >> information contained in this email is not authorized, and any such action >> should not be construed as a waiver of privilege or other confidentiality >> protections. >> >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 3 > Date: Thu, 21 Sep 2023 05:20:13 +0000 > From: John Kiernan <[email protected]> > To: Histonet <[email protected]>, Rhonda McCormick > <[email protected]> > Subject: Re: [Histonet] Long term museum specimen storage > Message-ID: > > <yt2p288mb0404405b06fabbc543a3ad1ba5...@yt2p288mb0404.canp288.prod.outlook.com> > > Content-Type: text/plain; charset="iso-8859-1" > > I don't know anything about "Jore's fixative" or the rationale of using a > very hypertonic unbuffered 4% formaldehyde with magnesium, sodium, chloride > and sulphate ions. If brown stuff is now bleeding out of your museum > specimens, Jore Juice evidently isn't a good preservative. > > According to Chapter 26 in the late Charles Culling's excellent book (3rd edn > 1974; ISBN: 0407729011) the fixative/preservative for a museum specimen is > optimized to preserve colour, which is the red or reddish-brown of > haemoglobin and myoglobin. This usually is achieved with Kaiserling's fluid, > which contains formalin, potassium acetate and also potassium nitrate (1.5% > w/v) as an oxidant. Another approach involves treating specimens with carbon > monoxide to convert all haemoglobin etc to a red carboxy derivative. > > If your museum specimens have already lost all their meaningful colours, a > neutral buffered aqueous formaldehyde may be the best that you can provide to > preserve the sizes and shapes. 70% alcohol will cause some shrinkage, and it > may not be as easy to seal this solvent into a museum container as a watery > diluted formalin. > > John Kiernan > = = = > ________________________________ > From: Rhonda McCormick via Histonet <[email protected]> > Sent: September 20, 2023 11:39 AM > To: Histonet <[email protected]> > Subject: [Histonet] Long term museum specimen storage > > Hi All, > I am looking to replace the fixative for veterinary specimens that have been > preserved as "museum specimens". They are kept in jars in a glass case > outside our lab, however, some of the fixative is starting to turn brown (and > we've pulled a few jars that have some slight cracks in them). > The specimens are currently in Jore's Fixative: 100 mL Distilled water > 10 mL 40% Formaldehyde2 g Magnesium Sulfate2 g Sodium Sulfate1 g Sodium > Chloride > Preserving specimens is new to me. I've never heard of Jore's fixative before > and I'm wondering if I could get some advice, please? Do these specimens > need to be replaced with the same solution? Could we rinse the specimen and > replace the solution with 70% Alcohol? OR would 10% NBF be better to store > the specimens in (or something al together different)? We have a varying > display of specimens - anywhere from a small porcine optic nerve to a large > equine granulosa cell tumor. Realizing it may be different based on the size > of the specimen, approximately how often should the solution be changed ? > Thank you so much! Any help or insight is much appreciated. > Rhonda McCormickRhonda McCormick BS, HT (ASCP)cm > Histology Diagnostic Lab Supervisor > > College of Veterinary Medicine > Texas A&M University > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 4 > Date: Thu, 21 Sep 2023 13:28:58 +0200 > From: "Gudrun Lang" <[email protected]> > To: 'Alonso Mart?nez Canabal' <[email protected]> > Cc: <[email protected]> > Subject: Re: [Histonet] Detergent in heating antigen retrieval > Message-ID: <[email protected]> > Content-Type: text/plain; charset="utf-8" > > Hi, > I think the answer is as so often: it depends. Detergens solves membranes > partly and leads to a higher permeabilization of the tissue. Some antigens > may take advantage of that, some may not need it. > Higher permeability is good for detection with high molecular complexes. > > Gudrun Lang > > -----Urspr?ngliche Nachricht----- > Von: Alonso Mart?nez Canabal via Histonet > [mailto:[email protected]] > Gesendet: Mittwoch, 20. September 2023 21:25 > An: Histonet > Betreff: [Histonet] Detergent in heating antigen retrieval > > Dear histoneters, > I have performed heating antigen retrieval with citrate buffer > pH 6 with 0.05% tween-20, however I have seem recipes with no detergent, > anyone has any experience or knowledge if it is better with or without the > detergent? > Thank you! > > -- > Dr. Alonso Mart?nez Canabal PhD > Profesor Asociado "C" > Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM > Investigador Nacional "I" > 56224833 > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Thu, 21 Sep 2023 12:40:40 -0400 > From: Amos Brooks <[email protected]> > To: "[email protected]" > <[email protected]> > Subject: [Histonet] p17 mice brain sections (Alonso Mart?nez Canabal) > Message-ID: > <CAC95ki8n2cLBoatYM5xNktGqOb=kk3t3udrd1li-qwpvs_n...@mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hi, > The thicker the section the more likely it will be to fall off. Frozen > sections already love to fall off slides. You should cut them a lot > thinner. 4 to 10 um should be the thickness, especially for frozens. > If you want very thick sections as you describe, you would be better > off cutting them and transferring them directly to an 8 well plate with PBS > and doing the IHC as a floating section. > > Cheers, > Amos Brooks > > >> >> Message: 1 >> Date: Tue, 19 Sep 2023 19:07:04 -0600 >> From: Alonso Mart?nez Canabal <[email protected]> >> To: Histonet <[email protected]> >> Subject: [Histonet] p17 mice brain sections >> Message-ID: >> <CAG12gaHPkXVjnHO= >> [email protected]> >> Content-Type: text/plain; charset="UTF-8" >> >> Hello, >> Have a great afternoon. I have done HAR with citrate buffer (pH 6.0) >> with tween, basically all my professional life of some 15 years. Several >> publications, using mice brain sections 40-50 microns thickness from >> cryostat (30% sucrose). Today I tried to do some p17 brain sections and the >> sections did not only fell off, but were completely destroyed. >> That never happened to me, I am wonder if anyone can have any idea of >> what happened? >> >> Thank you so much >> >> -- >> Dr. Alonso Mart?nez Canabal PhD >> Profesor Asociado "C" >> Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM >> Investigador Nacional "I" >> 56224833 >> > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 238, Issue 10 > ***************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
