Hi Histonetters

I’ve been trying to cut frozen fatty muscle tissue from 2018/2019 stored at 
-80C. I managed to cut some sections from the same tissue on fresh frozen 
tissue in 2018. I suspect that storage may contribute to the difficulty to cut 
this. I have yet touse dry ice to further lower the temperature. They are only 
small biopsies, but are heavily marbled.
Any ideas?

Gerard Spoelstra
 
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