If you want a stronger signal I would probably use the secondary antibody vs using a directly conjugated CD11c.
You could use a directly conjugated one as you would for flow cytometry but it won't give as much amplification as it would with a secondary step built in. Katy -----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: October-19-23 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 239, Issue 10 This email came from an EXTERNAL SENDER. If you think this message is suspicious, please do not open any attached files or links, and forward it as an attachment to spamm...@bccrc.ca Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IF staining questions (Charles Riley) ---------------------------------------------------------------------- Message: 1 Date: Thu, 19 Oct 2023 09:22:56 -0400 From: Charles Riley <cri...@udel.edu> To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IF staining questions Message-ID: <CAHMJNCjWAbcXgcHuzs657idSMpLhQEY=jks6ozooelwqdt3...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" Why would one decide to use a primary antibody along with a secondary antibody rather than a primary antibody conjugated with the secondary? Example. I have a researcher who wants to do CD11C staining with Alexafluor488 Is it better to buy and use a primary antibody CD11C conjugated with Alexafluor488 or to do the CD11C primary and a Rabbit anti-rat (H_L) IgG antibody secondary? ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 239, Issue 10 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
