I found the Histo-Logic archive on one of Sakura's web pages. Lee Luna was the editor for several years. Nothing there before about 1980 periodic acid for re-working H&E. The archive is huge and many contributions look good. See https://www.sakuraus.com/getattachment/103878b9-9854-469d-8203-9d9b1c45d850/761 but bear in mind that this is from a company. It's not a peer-reviewed scientific journal. John Kiernan. = = = ________________________________ From: Tony Henwood via Histonet <[email protected]> Sent: November 10, 2023 4:49 AM To: Akemi Allison <[email protected]>; jayalakshmy p.s <[email protected]> Cc: [email protected] <[email protected]> Subject: Re: [Histonet] Faded H&E tissue section
I thought he may have reported this in Histologic. This trick certainly works. You can notice the nuclear clarity on GIT and to a lesser extent skin biopsies stained with PAS. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children’s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: Akemi Allison<mailto:[email protected]> Sent: Friday, 10 November 2023 1:30 PM To: jayalakshmy p.s<mailto:[email protected]> Cc: Tony Henwood<mailto:[email protected]>; [email protected]<mailto:[email protected]> Subject: Re: [Histonet] Faded H&E tissue section Hi Tony: I actually gave that tip to Lee back in 1979 when he came to OHSU to give a seminar to the Oregon Histology Society. I found out that when I was doing GMA and the pathologists didn’t like Gills Hematoxylin. They loved my PAS on GMA so I tried using 1% Periodic Acid before using Harris Hematoxylin for H&E’s on GMA and it turned out beautifully. Guess he shared my tip with the rest of our society. He and I became closer se friends and we shared several tips, including his Movat’s tips which he didn’t publish, but I shared them in Frieda’s 4th Ed. Best, Akemi Allison-Tacha, BS, HT/HTL (ASCP) Sent from my iPhone > On Nov 9, 2023, at 6:11 PM, jayalakshmy p.s via Histonet > <[email protected]> wrote: > > Thanks, I'll check it out. > >> On Fri, Nov 10, 2023, 6:58 AM Tony Henwood <[email protected]> wrote: >> >> You could treat the decoverslipped section in 1% periodic acid (same as >> used in the PAS technique) for 30 minutes prior to H&E staining. This >> might improve the H&E staining. >> >> >> >> I believe Lee Luna suggested this but for the life of me, I can’t find the >> reference! >> >> >> >> Regards, >> >> >> >> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) >> >> Principal Scientist, the Children’s Hospital at Westmead (Retired) >> >> Adjunct Fellow, School of Medicine, University of Western Sydney. >> >> >> >> *From: *jayalakshmy p.s via Histonet <[email protected]> >> *Sent: *Friday, 10 November 2023 3:43 AM >> *To: *[email protected] >> *Subject: *[Histonet] Faded H&E tissue section >> >> >> >> Hello, >> I would like to know how to effectively restain a faded H&E tissue section. >> The color becomes dull when re stained. Somebody please advise. >> Prof. Jayalakshmy. P S >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
