Embed the bones diagonally in your molds if you're able (size depending) as this will allow for the greatest amount of paraffin support. Trim them very slowly, and if need be, place the blocks into the same "slow decal" solution for additional amount of time sufficient to enable better sectioning. Start checking in half hour or 45 minute intervals; rinse the blocks well in running water and attempt to section. If they're not ready, back into decal solution they go.
I feel that very wet ice helps to facilitate sectioning better than ice that is drier and fresh out of the freezer. Just be sure to blot the face of the block with gauze before attempting to cut. There's my two cents! Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edu -----Original Message----- From: Chakib Boussahmain via Histonet <histonet@lists.utsouthwestern.edu> Sent: Wednesday, January 24, 2024 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone samples (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-boun...@lists.utsouthwestern.edu. Hi guys, I hope this messagefinds you well. I am currently working on a study involving bone samples thathave been treated with slow decal and embedded in paraffin. I am facingchallenges in obtaining nice sections, and I was wondering if you could providesome guidance or recommendations. Thank you in advance for your help! Chakib _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://secure-web.cisco.com/1oOSMT1FuuHaA4EXxjY3i9nIAlLf91ZjdN5e2afysmGGJYW7stpUWSe1_bubEbbdKpoHohJt-UQ7lkDBZ_BwXbYaFsVfbmyQtwDLG4qorqorzByzCfrpkWZnOwj1ZW6oWKhNoWYgB3KojnS_P3jfgs8QItdqAV5QGAqwEewtholWiGRWxd-ggJXBstwd3TKwVx9EdGpjtjB5T3-MrhwB6gYGfUkRWiSu2yt6PL7FbWYjl11MtUyGiXP6EcnRSb4fQZzykJavCYeY0XmEMR6pe-rxP8W-MsmYb7NizNBFhqYFhAbuqVDhn5joQziAtv9KSW6AAMi1oI6aQW2ncTolPPVcXljMP586zzPK9P-9m9eF_j2JwX1WZjXV7N5Du2QCgAl5Rsz3xpqP0b9vzEXZqZ021FHX5HNuoA0X0iKq9tyKn6gc_8OwLotyO2g4bQTZa_FQuxStxGkGG1wOCjwntTrbvdllnoHTF4aKGZMFPiA0/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
