Hi, Since we have turned to embedding centers in the late 80ies we let the cassettes sit in the centers without additional paraffin. We only see such "jumping out" tissue, when the cassettes are not warmed (let the lid open) and the tissue renders too cold. As a result tissue and paraffin don't combine well enough. Maybe it is a matter of embedding technique? Too little paraffin in the mold before setting the tissue in? Cold embedding molds? Slow handling? Gudrun
-----Ursprüngliche Nachricht----- Von: Brazie, Jeneanne E *HS via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Freitag, 9. Februar 2024 11:41 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] tissue cassettes Hello :) I am encountering push back in our lab when I fill the embedding units with melted paraffin in the embedding wells. The techs here like for the tissue cassettes to sit dry (no wax) while in the embedding units. I find that the tissue rolls out of the sections while cutting because of a layering effect between the tissue and the paraffin its embedded in. I have communicated this but they tell me I'm "old school". Does anyone have any thoughts or opinions on this topic?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
