Thanks, Terri Me an OLD geezer too! I have always been an advocate of more time spent... meaningfully ( in-out of the dayjob) In my younger professional days.....I was criticised for proposing changes to techniques to minimise time spent on techniques eg: the Achest enzyme histochemical method for Hirschprung's disease ( use an antibody instead!); using DPX instead of Canada balsam (the de facto standard, then....) Who uses DPX nowadays???.....chuckle I disagree with you re " valuable time" Having spent many years as a Lab Manager....I know that there is always downtime This should be used to enhance technical know-how....eg: what is in the solutions/why do they work? For eg: it takes ~ 60 mins to make up Masson's reagents from basics These will last FAR longer than buying in expensive, low volume commercial preps Ditto Haemalum solutions...why use Gill's/Erlich's/Mayer's ?? We are teckies.....not RTU people? NB1: yep....places for RTU reagents NB2: it is interesting that Light sheet confocal microscopy for antibody use ....uses pretty much OLD techniques to "clear" the tissue Sure, different reagents but.....still a painfully LONG process? Discuss 😉
Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
