Hi John, A few common scenarios of why recoverslipping would be needed may give you the better response you're searching for. An example may be that the lab is diluting their coverslip medium too much and slides look great for a few days, or another scenario, where they forget to prime the tubing in their instruments or the viscosity is too thick, causing air to get dispensed rather than coverslipping medium.
Instead of a re-coverslipping percentage, what information are you looking for? Best wishes. Eddie Martin National Institutes of Health On Tue, Apr 29, 2025 at 1:00 PM <[email protected]> wrote: > Send Histonet mailing list submissions to > [email protected] > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > [email protected] > > You can reach the person managing the list at > [email protected] > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Removing H&E coverslippped slide (John O?Brien) > > > > ---------- Forwarded message ---------- > From: "John O’Brien" <[email protected]> > To: [email protected] > Cc: > Bcc: > Date: Mon, 28 Apr 2025 11:50:40 -0700 > Subject: [Histonet] Removing H&E coverslippped slide > > Histonetters > Can you experts in staining and Coverslipping offer the number of times > or % of slides recover slipped to a differ stain, any opinion is > appreciated > John > IMEB Inc > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
