Hi Manahil,

I look after our pathology products for Merck Life Science.

We have a great gentle ready to use decal (EDTA based) many people use for Bone 
Marrow Trephines which gives good results for when need to do molecular work.

It is one of our bestselling products in Australia.

The bone marrow floats to the top once reaches the end point too.

I am not sure if allowed to send product information here, please feel free to 
email me directly and I can give you more info if interested.


Regards



Georgina Gibson



Field Application Specialist – Tissue Diagnostics and Flavours & Fragrances

Life Science | Solution Scientists



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________________________________
From: MANAHIL EL BIREIR via Histonet <histonet@lists.utsouthwestern.edu>
Sent: Thursday, May 29, 2025 6:09:19 PM
To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Bone marrow trephine decalcification




I hope this email finds you well.



I am reaching  out regarding the optimisation of our bone marrow trephine 
specimen decalcification process. Currently, we are utilizing a ready-to-use 
acid rapid decalcification method. However, our Molecular Department has 
observed that the acid affects the quality of molecular results.



To enhance our process and ensure optimal molecular sequencing outcomes, we 
would greatly appreciate it if you could share your bone marrow trephine 
decalcification protocol with us. Additionally, which decalcification reagent 
you use to mitigate potential adverse effects on molecular analysis.



Kind regards,



Manahil





Sent from my iPhone

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