What type of slides are you using? At what temp and for how long are you baking the slides?
On Thu, Feb 12, 2026 at 11:00 AM <[email protected]> wrote: > Send Histonet mailing list submissions to > [email protected] > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > [email protected] > > You can reach the person managing the list at > [email protected] > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. GI biopsies (Paula) > > > > ---------- Forwarded message ---------- > From: Paula <[email protected]> > To: <[email protected]> > Cc: > Bcc: > Date: Wed, 11 Feb 2026 12:46:56 -0800 > Subject: [Histonet] GI biopsies > Dear Colleagues, > > I’m reaching out to the group to ask for input regarding microscopic folds > in GI biopsy sections. I’m hoping for some “tricks of the trade” > suggestions. > > In our lab, sections appear flat and well-relaxed on the water bath with no > visible gross folds. However, after H&E staining, the pathologist > identifies > epithelial microfolds in small GI biopsies (esophagus, stomach, colon). > These are not visible before staining and are occurring across multiple > techs. > > We currently use Leica Paraplast Plus (polymer-enhanced paraffin). We have > reviewed and optimized: > > • Embedding orientation (flat, mucosa aligned) > • Water bath temperature and float time > • Blade angle and cutting speed > • Section thickness (4 µm) > > > > Despite this, we continue to see post-stain microfolding. > > For those of you who cut a high volume of GI biopsies: > > · Have you found certain paraffins perform better (e.g., low-melt, > non-polymer)? > > · Do you run a different water bath temperature specifically for GI? > > · Any “tricks of the trade” for minimizing epithelial compression > that aren’t in the textbooks? > > I would greatly appreciate any practical advice or shared experience. Thank > you in advance for your insights. > > Best regards, > Paula Lucas > Histology Laboratory/BioPath Medical Group > > > > > > We are doing a lot more GI cases, and we’re experiencing complaints from > the > pathologist about folds. There are microscopic and are interfering with > taking images. > > > > Can someone recommend tricks to the trade of minimizing these folds in the > sections? > > > > Thank you in advance, > > Paula > > BioPath Medical Group > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
