I'm unqualified to speak about anything specific to horns, but I was once a 
metallurgist (PhD) with GE Aircraft Engines. Jet engines are carefully 
engineered not to "ring" for various reasons, especially that resonances can be 
VERY loud, and quickly lead to high cycle fatigue failure. But like horns, jet 
engines can make a beautiful sound (to an engineer). Unlike horns, the bell 
goes on the front. 

Anyway, the frequency of resonances are related to just two material properties 
- density and modulus of elasticity (a measure of inherent stiffness). Those 
two properties are not particularly sensitive to alloying, and are not affected 
at all by deformation processing, microstructure or heat treatment. What I'm 
saying is that a chunk of brass will resonate at a frequency that depends only 
on the shape of the chunk. The alloy, how it was formed, whether annealed or 
not will not make much difference. (It will matter if you're making a chime 
that must ring at 440 Hz exactly, but I don't think we're talking about this 
sort of resonance for the bell of a horn. Perhaps some rather some sort of very 
diffuse resonance.)

The stiffness of a bell will be greatly affected by wall thickness - different 
alloys and processes will produce different distributions of wall thickness 
within a bell. If two bells sound different, that's probably why. Annealing, 
cryo-treatments and that sort of thing will not affect the frequency of 
vibrations from a "thunk" or otherwise on a finished bell. Lacquer might, by 
adding weight and perhaps some stiffness. I can think of a possible exception: 
residual stresses could, I suppose, produce an affect similar to tightening the 
strings of a violin. "Annealing" is a bit of an all-purpose word - a heat 
treatment for this purpose would be called a "stress relief heat treatment".

If I've written the above paragraphs carefully, I've only referred to the 
*frequency* of vibrations, not their amplitude. The only mechanical property 
metallurgists use that relates to amplitude is the "damping factor". This is a 
measure of the energy absorbed internally by a metal when it vibrates. It is 
the property that would tell you how long a chime would ring - in a vacuum, 
that is. Compared to other forms of damping, it may be a small factor. The 
energy that is emitted into the air as sound is likely to be much greater. 

Engineers who design turbine airfoils desperately want them not to ring too 
loud, but generally don't care much about a material's damping factor; the 
energy to be dissipated is much too great - they rely on other means. Is 
damping factor important for horns? I would not presume to know. It is 
certainly important for instruments for which the instrument itself makes the 
air vibrate - cymbals, say. And unlike modulus of elasticity, damping factor 
can be influenced by various "extrinsic" parameters, such as impurity levels, 
microstructure - annealing, perhaps. It depends on little features in the metal 
that can act like shock absorbers/dampers. Steels have notably high damping 
factors - impurity atoms can flop about in its relatively open crystal lattice. 
In brass - I don't know - face-centered-cubic crystal structures don't allow 
much of that. Sorry I don't have much specific knowledge about brass.

I think it is safe to say that damping of a horn bell will be very strongly 
affected by contact with your hand. I can feel my bell vibrating if I hold it 
lightly. I can make greater contact with it if I want, and I sense that it does 
make a difference. I don't see much discussion about this factor. It wouldn't 
surprise me to learn that some people - perhaps without even knowing it - 
change their "grip" to produce different sounds. 


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