On Thursday, March 20, 2014 7:30:17 PM UTC+1, Mario Modesto wrote: > > Hello everybody, > > As the title sais, I recently obtained several images from a simple sample > with a microscope. And I would like to stitch them without deforming them. > How can it be done using Hugin? >
To put it simply, you want to stitch them in mosaic mode. For your input images, assume a rectilinear lens and assign an arbitrary horizontal field of view - just use a small value. The only parameters you should need to optimize are x and y, I don't think you should have much trouble with lens distortion but if you do you can try optimizing a, b and maybe c, but try without first. I would try using cpfind for the control points, possibly with a setting which will give you more control points than usual - try something like --fullscale --sieve2size 5 -o %o %s Output would be rectilinear, it shouldn't be difficult to get it to work, since you have so few parameters to play with. I do assume you are using a mechanical stage, if not you need to optimize for r (roll) as well. And you shouldn't refocus from one image to the next, because that will confuse the control point generator, which needs identical bits of image to recognize, and with the very small depth of field of microscopic images a small change in focus may make it impossible for the cpg to do it's job - the problem will be more pronounced with higher magnifications. One more thing: make sure your microscope is köhlered precisely - see http://en.wikipedia.org/wiki/K%C3%B6hler_illumination Kay > Thanks > Mario > -- A list of frequently asked questions is available at: http://wiki.panotools.org/Hugin_FAQ --- You received this message because you are subscribed to the Google Groups "hugin and other free panoramic software" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To view this discussion on the web visit https://groups.google.com/d/msgid/hugin-ptx/de89f6a0-2e06-4572-8a72-aadfa656559f%40googlegroups.com. For more options, visit https://groups.google.com/d/optout.
