Maybe this task is similar to what is described in the Hugin tutorial — 
Stitching murals using mosaic mode 
<http://hugin.sourceforge.net/tutorials/Mosaic-mode/en.shtml> but I question if 
your microscopy needs also require deconvolution microscopy. I suppose the 
deconvolution process needs to occur prior to Hugin stitching. The stitching 
results at the image boundaries may not be suitable for your needs without 
doing some tight masking. It would be interesting to see the results you come 
up with. 
> On Mar 30, 2020, at 3:37 AM, Quantrix <[email protected]> wrote:
> 
> Hi there,
> I am looking to solve a similar problem using microscopy images. I was 
> wondering if anyone had any inputs, insights and guidance regarding this 
> issue?
> Thanks
> Quantrix
> 
> On Friday, 13 March 2020 00:13:30 UTC-6, Ahron wrote:
> Hi,
> 
> I have a macro scanner made out of basically smooshing a USB microscope into 
> the extruder hole of a 3D printer. Said USB microscope captures 480x720 
> images in a regular grid of equal overlaps of ~20-50%. The mechanical 
> repeatability of the system is good to better than or no more than a few 
> pixels, depending on magnification. Is there a set of settings for this 
> scenario with Hugin? I feel like it should be almost "easy mode", but in my 
> current state of things (mostly running the auto and fumbling with controls) 
> I can't seem to get a good picture with anything! 
> 
> I currently use microsoft ICE since it usually does a fantastic job with no 
> muss or fuss, but have recently been looking for an alternative for reasons 
> involving lack of API and formation of artifacts. I am sure you have heard 
> this story somewhere before, but right now to do batching we have an open 
> loop VBS script that runs through the menus with physical keypresses!
> 
> Beyond the actual stitching them together, I am sure there is plenty to deal 
> with blending, dealing with exposure changes, etc... my hope is that by 
> controlling many variables though I will be able to automate the process to 
> minimal human input most of the time. Does anyone in the community have faith 
> that this is possible? it would mean a great deal to me, as my project means 
> a great deal to me.
> 
> Thank you so much for any possible assistance!
> 
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