I do some pretty fancy image manipulation and I use numpy + PIL to do my 
processing. What you are describing can easily be achieved by using those 
packages together.




________________________________
From: Chuck Pepe-Ranney <cpep...@mines.edu>
To: image-sig@python.org
Sent: Monday, September 21, 2009 2:28:04 PM
Subject: [Image-SIG] manipulating image for counting microbial cells

Hello,
I am wondering if I can use PIL to manipulate some fluorescent microscopy 
images.  Specifically, I will have two images of the same field but each will 
be taken under different emission filters.  What I need to do is loop through 
the pixels in the first image multiply each pixel's intensity by a factor then 
divide it by the intensity of the spatially corresponding pixel in the second 
image.  There are more steps to follow but they are seemingly routine image 
manipulation tasks.  The images will be in tiff format.  Would PIL be a 
suitable solution to this problem?
Thanks for the help in advance,
- Chuck



      
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