> in Fiji > we consider pixel coordinates at a pixel's center which is in perfect > agreement when working with microscopic images (particularly confocal > scanners where the coordinate really is a point sample
It's not really - the scanned point is normally moving for the duration of sampling, so there is some lateral smear of the sampled space. In many confocals the scan speed varies within a row, so the 'point samples' aren't even the same on that level. True point-by-point scanning requires the scan mechanism to dwell for the duration of measurement, then not measure while the mechanism moves to the next dwell point. As far as I'm aware this is not implemented in current 'point-scanners' because it would be horribly slow to stop and start the scan mirrors millions of times per frame. Michael _______________________________________________ ImageJ-devel mailing list [email protected] http://imagej.net/mailman/listinfo/imagej-devel
