Hi Michael,

which command do you refer to?
Image>Stacks>3D Project does keep the spatial calibration in the current version of ImageJ. Example macro:

  run("T1 Head (16-bits)");
run("3D Project...", "projection=[Brightest Point] axis=X-Axis slice=1.50 initial=0 total=360 rotation=10 lower=1 upper=255 opacity=0 surface=100 interior=50");

(mind possible line breaks introduced by the mailer; the macro should have two lines only)


Michael
________________________________________________________________
On 02.04.24 20:24, Cammer, Michael wrote:
I've gotten used to projections of stacks with spatial scales assigned losing 
the spatial scales.  The resulting rotations are scaled in pixels.

However, I recently used an old version (years ago) of ImageJ installed on 
someone else's confocal and a resulting 3D projection did have the spatial 
scale included and correct in XY.

Is the loss of the spatial scale intentional or a bug?

Cheers-

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

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