Hmm - I’ll have to test again.  I use ImagePlus.resize() from within a Java
plugin and didn’t see the Calubration change.

I’ll try again.  Question - how did you change the dimensions?  From the
GUI?

-Kenneth Sloan


On Fri, Aug 16, 2024 at 07:19 Straatman, Kees (Dr.) <[email protected]>
wrote:

> Hi Kenneth,
>
> Not sure what you mean. If I open Confocal Series I see
> pixel_width=0.0544550 pixel_height=0.0544550. If I change the image size
> from 400 pixels to 200 pixels I get pixel_width=0.1089100
> pixel_height=0.1089100, so the calibration has been adjusted.
>
> Best wishes
> Kees
>
> Advanced Imaging Facility (RRID: SCR_020967)
>
> University of Leicester
>
> www.le.ac.uk/advanced-imaging-facility<
> http://www.le.ac.uk/advanced-imaging-facility>
>
>
> ________________________________
> From: Kenneth Sloan <[email protected]>
> Sent: 15 August 2024 02:16
> To: [email protected] <[email protected]>
> Subject: Calibration feature request
>
> ***CAUTION:*** This email was sent from an EXTERNAL source. Think before
> clicking links or opening attachments.
>
> I have been making more and more use of the Calibration feature to
> establish custom coordinate systems for images.  I note that when an image
> is re-sized (and presumably cropped, although I haven't tested this yet),
> the Calibration remains the same.
>
> Is it possible for operations such as "resize" to update the Calibration,
> if one is attached to an image?  It seems obvious to me (perhaps it's not
> obvious?) that xOrigin, yOrigin, pixelHeight, pixelWidth can all be
> modified in a straightforward way.
>
> --
> Kenneth Sloan
> [email protected]
> Vision is the art of seeing what is invisible to others.
>
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