Hi Dipanjana, Here is an example Jython script that segments and measures puncta in 3D:
https://ops.scijava.org/en/1.1.0/examples/volume_labeling.html The steps involved are spelled out in the tutorial narrative as well as source code comments, so hopefully you can figure out how to adapt it to your specific use case. The docs (https://imagej.net/scripting), Image.sc Forum (https://forum.image.sc/), this mailing list, and LLMs like https://bioimage.io/chat and ChatGPT can all help your learn more. Regards, Curtis On Sat, Aug 17, 2024, 01:07 Dipanjana Ghosh <[email protected]> wrote: > Dear all, > > We want to calculate the intensities of a channel where objects (puncta) > are detected. The intensities from the puntas are to be calculated applying > some threshold for size and intensities. > Can someone kindly explain which tool of imageJ can be used for this and > how? > > Thanking you in advance > > Regards, > Dipanjana > > -- > Dr. Dipanjana Ghosh > DST INSPIRE Faculty > School of Biomolecular Engineering & Biotechnology > RGPV, Bhopal > ORCID: https://orcid.org/0000-0003-0887-6798 > Prev: DST WOS-A, IISERB > Post doctoral Research Fellow > Department of Biological Sciences > National University of Singapore > Singapore > > -- > ImageJ mailing list: http://imagej.nih.gov/ij/list.html > -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html
