Greetings Dmitry,
in order to be able to substantially provide some help, we need to see
typical images in a non-lossy file-format, preferably TIF .
"advice on how to address defocus in these images"
There is only one generic method which is "(adaptive) Wiener-corrected
inverse filtering". AI-approaches *at best* will produce comparable results.
Building good inverse filters is far from trivial because they strongly
depend on the individual image statistics (S/N-ratio):
<https://www.gluender.de/Writings/WritingsTexts/WritingsDownloads/1980_NoiseInDeblurring_scan.zip>
Regards
Herbie
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Am 28.11.24 um 19:16 schrieb Dmitry Semchonok:
Dear colleagues,
I hope this message finds you well.
I am seeking guidance on the proper methods for analyzing liposomes in
cryo-electron microscopy (cryo-EM) images. Specifically, I have some raw
cryo-EM images (*.mrc, *.eer) containing liposomes, and I would like to
determine their diameter, area, and circularity etc.
Is there a batch script available that can facilitate the measurement of these
parameters and assist in preparing the necessary statistics?
Additionally, I would appreciate any advice on how to address defocus in these
images.
Thank you for your assistance.
Kind regards,
Dmitry
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