Dear Leoncio, If you want to use FLIMJ you can also try to record the movement of the mouse and control of Keyboard for fields. I am aware of two plugins that can do this: IJRobot: https://imagej.net/plugins/ij-robot AutoClickerJ: https://github.com/pmascalchi/ImageJ_AutoClickerJ
I have used IJ Robot successfully for several plugins that where not recordable. Best wishes Kees Advanced Imaging Facility (RRID: SCR_020967) University of Leicester http://www.le.ac.uk/advanced-imaging-facility -----Original Message----- Sent: 31 March 2025 15:43 Subject: Re: running FLIMJ from macro or script [Some people who received this message don't often get email from [email protected]. Learn why this is important at https://aka.ms/LearnAboutSenderIdentification ] ***CAUTION:*** This email was sent from an EXTERNAL source. Think before clicking links or opening attachments. Hi Leoncio, The current version of FLIMJ does not currently support a batch mode. Unfortunately, trying to use the macro recorder will only produce a useless macro to start FLIMJ, but not one that could be used to script its function. There are two main ways to do batch processing using Fiji and FLIMLib: 1. Use flimj-ops: FLIMJ uses flimj-ops and FLIMLib to perform the fitting. You can use flimj-ops directly in a Fiji script to perform batch processing on your data. a. Here's the flimj-ops GitHub repository: https://github.com/flimlib/flimj-ops b. Here's a link to the flimj-ops notebooks where you can see how to use it in a script for fitting data: https://github.com/flimlib/flimj-ops/tree/master/notebooks 2. Use SciJava Ops: We recently published our new image processing/algorithm framework where you can use the same FLIMLib library that FLIMJ uses to fit your data. a. Here's a link to our FLIM analysis use case using SciJava Ops and Fiji: https://ops.scijava.org/en/latest/examples/flim_analysis.html I hope that helps. Please let me know if you have any other questions. -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html
