Absolutely!
Actually, "Absolutely not" is the correct answer here. :)
Some anti-spam programs check for the lack of a reverse DNS entry, others will also verify that the hostname in the reverse DNS entry has an A record pointing back to the same IP. But, extremely few (1 in 10,000 mailservers perhaps) will do anything beyond that, because it just isn't effective.
AOL will definitely care if you don't have a reverse DNS entry, probably care if you have a reverse DNS entry that doesn't point back to your mailserver, but it won't care at all if your HELO/EHLO doesn't match the reverse DNS entry.
-Scott
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