Hi Tanay, As you said, they could have cited the reference for the primers. But matK is a quite conservered region and some papers even suggest it to be a suitable region for DNA barcoding like coxI for animals. Manytimes a partial matK region is enough for phylogeny. I am sure, matK would be accurate upto the genus level, but after getting the clue we can look for morphological similarity between the Agave sisalana stem and Ramkhand for further clarification.
Sid. On Thu, May 12, 2011 at 7:56 PM, tanay bose <[email protected]> wrote: > The interesting but If you are using PCR in your experiment then > you should always mention the "primers" used. The paper seriously > lack the information. Next with partial amplification of a gene you > cant be sure that its from *A sisalana* !! > Tanay > > On Wed, May 11, 2011 at 11:05 PM, Ritesh Kumar Choudhary < > [email protected]> wrote: > >> >> Just saw this article in Current Science. >> >> Regards, >> Ritesh. >> >> > > > -- > *Tanay Bose* > Research Assistant & Teaching Assistant. > Department of Botany. > University of British Columbia . > 3529-6270 University Blvd. > Vancouver, B.C. V6T 1Z4 (Canada) > Phone: 778-323-4036 (Mobile) > 604-822-2019 (Lab) > 604-822-6089 (Fax) > [email protected] > *Webpages:* > http://www.botany.ubc.ca/people/mberbee.html > http://www.botany.ubc.ca/people/gradstud.html > https://sites.google.com/site/efloraofindia/ > > >
