Hi,

I used Jalview to perform principle component analysis, but it said "Out of 
memory when calculating PCA!! See help files for increasing Java Virtual 
Machine memory". Then I see help files 
(http://www.jalview.org/jvmmemoryparams.html). It told me to add two lines into 
Jalview.lax:

lax.nl.java.option.java.heap.size.max=1000m
lax.nl.java.option.java.heap.size.initial=500m
When I opened the file Jalview.lax. I found that there were already two lines 
which showed:
lax.nl.java.option.java.heap.size.initial=33554432 
lax.nl.java.option.java.heap.size.max=268400000 
So my question is whether we should add two lines to the end of this file? The 
default memory is larger than your suggested memory, if we change or add these 
two lines, can we fix 
this probelm? Even we added these two lines, the problem was not fixed, it 
still said "Out of memory when calculating PCA!! See help files for increasing 
Java Virtual Machine memory".So what
is the problem and how to fix it?
Thanks in advance!
Best wishes!
Yizhuang Zhou
2017-01-06 



Yizhuang Zhou 
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