Hi Ben/Jeff,

Thank you for getting back to me. If I load an existing project/alignment, 
colour by conservation works. However, if in the same project I generate a new 
alignment from a set of fasta sequences, it doesn’t. The formatting of the new 
alignment is also different: lines are now wrapped and font type/size has 
changed.

Best wishes,
Luca
 
Luca Pellegrini, PhD
Department of Biochemistry
University of Cambridge
Cambridge CB2 1GA
UK



> On 17 May 2021, at 16:37, Geoffrey Barton (Staff) <g.j.bar...@dundee.ac.uk> 
> wrote:
> 
> Hi Luca,
>  
> Sorry you are having trouble with this.
>  
> I just tried it with a Pfam alignment and it seemed to work OK for me both 
> conservation shading and group shading by conservation.
>  
> I have the same version and  macOS as you.     Some things to try are:  
> reselect the colour by conservation option and select the “modify 
> conservation threshold” option as well.  Sometimes selecting a different 
> colour scheme, then going back to clustalX/conservation colouring fixes 
> things.
>  
> Mmm, are you talking about conservation on a linked structure?   If so, I 
> will give that a try too.
>  
> Geoff.
>  
> -- 
> Geoff Barton FRSE FRSB | Professor of Bioinformatics | Head of Division of 
> Computational Biology  
> School of Life Sciences | University of Dundee | Scotland, UK | email: 
> g.j.bar...@dundee.ac.uk <mailto:g.j.bar...@dundee.ac.uk>
> Web: www.compbio.dundee.ac.uk <http://www.compbio.dundee.ac.uk/> | twitter: 
> @gjbarton and @bartongrp
>  
>  
> From: jalview-discuss-boun...@jalview.org 
> <mailto:jalview-discuss-boun...@jalview.org> 
> <jalview-discuss-boun...@jalview.org 
> <mailto:jalview-discuss-boun...@jalview.org>> on behalf of Luca Pellegrini 
> <lp...@cam.ac.uk <mailto:lp...@cam.ac.uk>>
> Date: Monday, 17 May 2021 at 16:01
> To: Jalview-discuss@jalview.org <mailto:Jalview-discuss@jalview.org> 
> <Jalview-discuss@jalview.org <mailto:Jalview-discuss@jalview.org>>
> Subject: [Jalview-discuss] Colour by conservation problem
> 
> Hello,
> 
> 'Colour by conservation’ with Clustal colouring has stopped working. Can 
> anyone please advise? I’m using Jalview v2.11.1.4 and macOS Catalina.
> 
> Best wishes,
> Luca
> 
> Luca Pellegrini, PhD
> Department of Biochemistry
> University of Cambridge
> Cambridge CB2 1GA
> UK
> 
> 
> 
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