Miguel wrote:

I make a mistake with the first 10 positions:-(
and the naming of N looks strange " N A" though the N is on the right
position.
and HEM546.O1A is identified as oxygen by Jmol, which is correct



This is the key to the answer.

This formula spans two lines. This is the first time that I have ever seen
a formula that took more than one line. The code is only taking the second
FORMUL record for HEM ...



123456789.12Xx__6789.123456789.123456789.123456789.123456789.123456789.123456Xx
FORMUL 4 HEM C34 H32 N4 O4 FE1 ++ .
1MBO 63
FORMUL 4 HEM O2



This one is overriding the previous FORMUL definition for HEM

Q: Why are there 2 definitions for HEM? Is it because the HEM can carry
two oxygens in its oxidized state?


this was an ugly PDB annotation, and it is not correct, because there is no continuation number in 17 & 18

the molecular O2 should better be an independent group like with 1f65
http://www.imb-jena.de/cgi-bin/SCOPlnk.exe?JMOL=1f65

Q: What is the meating of the ++ and the . in the first formula definition
for HEM?


I guess the charge from Fe 2+ and an electron which was donated from the basic HIS93 and because Fe 2++ removes the to central protons of the porphyrin leaving 2- to be delocalized ... don't discuss the side chains and delocalization at O2 1- .. Fe 3+
would result in a overall negative charge ?


((C34 H32 N4 O4 2-) (FE1 2+) 1-)

Regards, Jan


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