Hello Molly,

There are a few things that could be going on here.  What method are
you using to communicate between your VI's?  I will assume you are
using serial.

If a port is not open, the port will discard the message, which could
be what is happening here.  If you are trying to write from computer A
to B and then have B respond back to A, your code should look like
this:

Computer A:
Open VISA serial port.
Wait until computer B has the program running and has opened its VISA
port (there is no programmatic way to do this, you will need to
synchronize this by hand).
Send request.
Read response.
Close port.

Computer B:
Open VISA serial port.
Read request.
Send response.
Close port.

Hope this helps.  If you need more assistance, feel free to post here.

Scott B.
Applications Engineer
National Instruments

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