Dear marine mammal community,
A new paper about Platelet-Rich Plasma and Adipose-Derived Mesenchymal Stem
Cells for Regenerative Medicine-Associated Treatments in Bottlenose Dolphins
(Tursiops truncatus) has recently been published.
Citation:
Griffeth RJ, García-Párraga D, Mellado-López M, Crespo-Picazo JL,
Soriano-Navarro M, et al. (2014) Platelet-Rich Plasma and Adipose-Derived
Mesenchymal Stem Cells for Regenerative Medicine-Associated Treatments in
Bottlenose Dolphins (Tursiops truncatus). PLoS ONE 9(9): e108439.
doi:10.1371/journal.pone.0108439
Abstract:
Dolphins exhibit an extraordinary capacity to heal deep soft tissue injuries.
Nevertheless, accelerated wound healing in wild or captive dolphins would
minimize infection and other side effects associated with open wounds in marine
animals. Here, we propose the use of a biological-based therapy for wound
healing in dolphins by the application of platelet-rich plasma (PRP). Blood
samples were collected from 9 different dolphins and a specific and simple
protocol which concentrates platelets greater than two times that of whole
blood was developed. As opposed to a commonly employed human protocol for PRP
preparation, a single centrifugation for 3 minutes at 900 rpm resulted in the
best condition for the concentration of dolphin platelets. By FACS analysis,
dolphin platelets showed reactivity to platelet cell-surface marker CD41.
Analysis by electron microscopy revealed that dolphin platelets were larger in
size than human platelets. These findings may
explain the need to reduce the duration and speed of centrifugation of whole
blood from dolphins to obtain a 2-fold increase and maintain proper morphology
of the platelets. For the first time, levels of several growth factors from
activated dolphin platelets were quantified. Compared to humans, concentrations
of PDGF-BB were not different, while TGFβ and VEGF-A were significantly lower
in dolphins. Additionally, adipose tissue was obtained from cadaveric dolphins
found along the Spanish Mediterranean coast, and adipose-derived mesenchymal
stem cells (ASCs) were successfully isolated, amplified, and characterized.
When dolphin ASCs were treated with 2.5 or 5% dolphin PRP they exhibited
significant increased proliferation and improved phagocytotic activity,
indicating that in culture, PRP may improve the regenerative capacity of ASCs.
Taken together, we show an effective and well-defined protocol for efficient
PRP isolation. This protocol alone or in
combination with ASCs, may constitute the basis of a biological treatment for
wound-healing and tissue regeneration in dolphins.
You can download it for free at:
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0108439
I hope you find it interesting.
Best regards
Daniel
_________________________________________________________
Daniel García Párraga, DVM
Director
of Veterinary and Laboratory Services.
Biology Department.
Oceanogràfic. Grupo Parques Reunidos. Ciudad de las Artes y las Ciencias.
________________________________________________________________
Telf:
(+ 34) 96 1975501
Telf direct: (+ 34) 96 1975508
Fax
: (+34) 96 1975511
Oceanogràfic. Grupo Parques Reunidos Valencia.
C/.
Eduardo Primo Yúfera (Científic), nº 1B. 46013. Valencia. Spain.
e-mail: [email protected]
web: www.cac.es/ www.parquesreunidos.com
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