Dear Colleagues,
We are pleased to announce the publication entitled:

"Respiratory Microbiome of Endangered Southern Resident Killer Whales and 
Microbiota of Surrounding Sea Surface Microlayer in the Eastern North Pacific"

Stephen A. Raverty, Linda D. Rhodes, Erin Zabek, Azad Eshghi, Caroline E. 
Cameron, M. Bradley Hanson & J. Pete Schroeder
The publication is available through the link below.

http://www.nature.com/articles/s41598-017-00457-5

Abstract
In the Salish Sea, the endangered Southern Resident Killer Whale (SRKW) is a 
high trophic indicator of ecosystem health. Three major threats have been 
identified for this population: reduced prey availability, anthropogenic 
contaminants, and marine vessel disturbances. These perturbations can culminate 
in significant morbidity and mortality, usually associated with secondary 
infections that have a predilection to the respiratory system. To characterize 
the composition of the respiratory microbiota and identify recognized pathogens 
of SRKW, exhaled breath samples were collected between 2006-2009 and analyzed 
for bacteria, fungi and viruses using (1) culture-dependent, targeted PCR-based 
methodologies and (2) taxonomically broad, non-culture dependent PCR-based 
methodologies. Results were compared with sea surface microlayer (SML) samples 
to characterize the respective microbial constituents. An array of bacteria and 
fungi in breath and SML samples were identified, as well as microorganisms that 
exhibited resistance to multiple antimicrobial agents. The SML microbes and 
respiratory microbiota carry a pathogenic risk which we propose as an 
additional, fourth putative stressor (pathogens), which may adversely impact 
the endangered SRKW population.

Kindest regards,
Stephen Raverty
Veterinary Pathologist, AHC, BC MAL
Abbotsford, BC
[email protected]

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