Dear all, My co-authors and I are pleased to announce the new publication of the following article in Scientific Reports.
Wen-Ta Li, Hui-Wen Chang, Wei-Cheng Yang, Chieh Lo, Lei-Ya Wang, Victor Fei Pang, Meng-Hsien Chen & Chian-Ren Jeng *Immunotoxicity of Silver Nanoparticles (AgNPs) on the Leukocytes of Common Bottlenose Dolphins (Tursiops truncatus)* Silver nanoparticles (AgNPs) have been extensively used and are considered as an emerging contaminant in the ocean. The environmental contamination of AgNPs is expected to increase greatly over time, and cetaceans, as the top ocean predators, will suffer the negative impacts of AgNPs. In the present study, we investigate the immunotoxicity of AgNPs on the leukocytes of cetaceans using several methods, including cytomorphology, cytotoxicity, and functional activity assays. The results reveal that 20 nm Citrate-AgNPs (C-AgNP20) induce different cytomorphological alterations and intracellular distributions in cetacean polymorphonuclear cells (cPMNs) and peripheral blood mononuclear cells (cPBMCs). At high concentrations of C-AgNP20 (10 and 50 μg/ml), the time- and dose-dependent cytotoxicity in cPMNs and cPBMCs involving apoptosis is demonstrated. C-AgNP20 at sub-lethal doses (0.1 and 1 μg/ml) negatively affect the functional activities of cPMNs (phagocytosis and respiratory burst) and cPBMCs (proliferative activity). The current study presents the first evidence of the cytotoxicity and immunotoxicity of AgNPs on the leukocytes of cetaceans and improves our understanding of environmental safety concerning AgNPs. The dose-response data of AgNPs on the leukocytes of cetaceans are invaluable for evaluating the adverse health effects in cetaceans and for proposing a conservation plan for marine mammals. Free access to the article is available at https://www.nature.com/articles/s41598-018-23737-0 orPDF by email at this address heerolee1...@gmail.com or via researchgate (here <https://www.researchgate.net/publication/324212086_Immunotoxicity_of_Silver_Nanoparticles_AgNPs_on_the_Leukocytes_of_Common_Bottlenose_Dolphins_Tursiops_truncatus> ) Cheers, Wen-Ta Li -- Wen-Ta Li, DVM, MS PhD candidate/Veterinary Pathologist Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University Veterinarian/Director, Taiwan Cetacean Society
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