Dear MARMAM community,
My coauthors and I are excited to announce our recent paper on hormone analyses in small blubber samples (50 mg) of gray and fin whales published in Frontiers in Marine Science. It is open access and can be found here: https://www.frontiersin.org/articles/10.3389/fmars.2021.808764/full Wittmaack C, Urbán Ramírez J, Bernot-Simon D, Martínez-Aguilar S, Subbiah S, Surles JG, Looney M, Kumar N, Halaska B, Duignan PJ, Knauss M, Burns K and Godard-Codding CAJ (2022). Small Blubber Samples (50 mg) Sufficient for Analyses of 10 Stress and Reproductive Steroid Hormones in Gray and Fin Whales via Liquid Chromatography Mass Spectrometry. Front. Mar. Sci. 8:808764. doi: 10.3389/fmars.2021.808764 ABSTRACT Information on stress, reproductive fitness, and health is difficult to obtain in wild cetaceans but critical for conservation and management. The goal of this study was to develop a methodology requiring minimal blubber mass for analysis of reproductive and stress steroid hormones and, hence, suitable for cetacean biopsies. Blubber biopsies and samples were collected from free-ranging and stranded gray and fin whales. Steroid hormones were extracted from blubber samples as small as 50 mg using liquid-liquid extraction methodology developed to handle the high fat content of blubber. Samples were analyzed via liquid chromatography with tandem mass spectrometry for 10 hormones: aldosterone, androstenedione, cortisol, cortisone, corticosterone, 17b-estradiol, estrone, 17a-hydroxyprogesterone, progesterone, and testosterone. As part of the optimization, homogenization via bead beating and blade dispersion were compared, and the former found superior. To investigate optimal yet minimal tissue mass required, hormone panels were compared among paired 50, 150, and 400 mg samples, the latter two being commonly reported masses for hormone blubber analysis. Results indicated that 50 mg of blubber was suitable and sometimes superior. Additionally, significant differences in precision values were observed between species, possibly stemming from differences in blubber composition, and relevant to homogenization technique selection and calibration methods that use blubber matrix matches obtained from a species other than the study species. Based on recovery and precision values, our methodology was accurate and precise in the measurement of spiked known quantities for all 10 hormones, confirming the methodology capabilities in 50 mg blubber mass in both species. Altogether, and in our specific sample sets, all endogenous hormones, except corticosterone, were identified above the detection limit in 50 mg gray whale blubber samples while all endogenous hormones, except aldosterone, cortisone, Please feel free to contact me at [email protected] Best, Céline Godard-Codding ***************************************************************** Céline Godard-Codding, Ph.D. Professor and Associate Chair Department of Environmental Toxicology The Institute of Environmental and Human Health Texas Tech University and TTU Health Sciences Center Box 41163 Lubbock, Texas 79409-1163 Office: (806)-834-2737 Fax: (806)-885-2132 FEDEX shipping address: The Institute of Environmental and Human Health (TIEHH) Reese Technology Center Building 555 1207 S. Gilbert Drive Lubbock TX 79416-2103 *****************************************************************
_______________________________________________ MARMAM mailing list [email protected] https://lists.uvic.ca/mailman/listinfo/marmam
