Hi Karen, Mauve's GenBank parser is peculiarly picky about the format of the file's header. But the good thing is that once you figure out what is "off" about your file, you don't need to rerun the alignment -- since the sequence is unchanged, just overwrite the original gbk file with your edited one, and open the alignment in Mauve.
(1) I'm not sure what the issue with the RAST file is -- I just tested a couple of recent RAST files of mine, and their annotations load fine. If you cannot figure it out by comparing it to a working file, I'd be glad to take a look at your file if you want. (2) Mauve pulls the display name from the first source Feature's /organism qualifier, and appends the /strain qualifier UNLESS that text already appears in the /organism text. It appears that /sub_strain, /plasmid, etc., are ignored, but editing /organism and/or /strain allows you to control the labels displayed in the alignments ... For your specific case, you could just change /organism to "Klebsiella pneumoniae pKPoxa-48N1" (should be in double quotes). Cheers, Guy Dr. Guy Plunkett III Senior Scientist, Genome Center of Wisconsin Senior Scientist, DNASTAR http://www.genome.wisc.edu/information/gplunkett.html On 10/25/13, karen power wrote: >Hi, > >I am using MAUVE to compare a RAST annotated plasmid sequence and a gbk file >from genbank. The gbk file is accession HF955507. However the RAST-annotated >file will not load annotations. I tried removing the space after 'taxon' as >suggested here previously but this did not make a difference. I would also >like to know how to change the file name so that within the MAUVE window is >comes up as 'Klebsiella pneumoniae pKPoxa-48N1' instead of just 'Klebsiella >pneumoniae' as it is now. > >Can anyone help? > >Kind regards, > >-- ------------------------------------------------------------------------------ October Webinars: Code for Performance Free Intel webinars can help you accelerate application performance. Explore tips for MPI, OpenMP, advanced profiling, and more. Get the most from the latest Intel processors and coprocessors. See abstracts and register > http://pubads.g.doubleclick.net/gampad/clk?id=60135991&iu=/4140/ostg.clktrk _______________________________________________ Mauve-users mailing list Mauve-users@lists.sourceforge.net https://lists.sourceforge.net/lists/listinfo/mauve-users
