Hello everyone,
I have a question about negative values on backbone files.
My question is: is the negative value the coordinate to the complementary
strand 5'->3'? Or it is the absolute value of complementary strand 3'->5'?
Lets say i have a sequence that ranges from 1 to 100 nt. If my backbone
file points -20 to -30 it is equivalent to the region 70 to 80 of the
complementary strand?
I want to know because i'm crossing coordinates with gbk files, in which
the complementary is counted from the end to the beggining (absolute value
5'->3'), to check if there is overlaps and i'm very confused on how it
works...
Example:
5' ATCATCGCAT '3
'3 TAGTAGCGTA '5
If the backbone points -2 to -4 it's "AGTA" (absolute value 3'->5') or it
is "TGCG" (absolute value 5'->3').
In other words to get the same coordinates of a gbk file i have to subtract
the absolute value to the genome lenght in order to obtain the real
coordinate?
Thanks in advance,
*Eddie Imada*
*Eddie Imada*
Mestrando em Genética e Biologia Molecular - UEL
Laboratório de Genética de Microorganismos
Cel: (43) 9973-3416
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