Hi all,

I tried running this command again not using our server's queuing system and got a slightly more informative error. Any ideas?

uqkkocot@barrine3:~> progressiveMauve --seed-family --seed-weight=11 --backbone-output=backbone_output --output=output.mauve seq1 Amphimedon_queenslandica_genomic_scaffolds.fa seq2 Mnemiopsis_leidyi_genomic_scaffolds.fa seq3 Monosiga_brevicollis_genomic_scaffolds.fa seq4 Nematostella_vectensis_genomic_scaffolds.fa seq5 Pleurobrachia_bachei_genomic_scaffolds.fa seq6 Trichoplax_adhaerens_genomic_scaffolds.fa
Exception FileNotOpened thrown from
Unknown()  in gnFileSource.cpp 67
Called by Unknown()

Searching with seed pattern 111001001010100100111

Searching with seed pattern 1110101001001010111

Searching with seed pattern 1111001010101001111
done
Floating point exception

Thanks!
Kevin

On 07/06/14 15:34, Kevin Kocot wrote:
Hi all,

I'm interested in examining genomic synteny among several different animal phyla but I'm getting an error I don't understand. I'm not sure if this is an issue with progressiveMauve itself or my cluster (The University of Queensland's Barrine server). Also, I was wondering if anyone has any thougths on the initial settings I'm trying and if you think these would be appropriate for this case. I expect a high degree of genomic re-arrangement among these taxa and, in some cases, a lot of variation in sequence.

_The script I'm running:_
#!/bin/bash
#PBS -N Mauve -A uq-Biology -m ea -M k.ko...@uq.edu.au
#PBS -l walltime=300:00:00
#PBS -l select=1:ncpus=8:NodeType=large:mem=72g
module load mauve/2.3.1
cd $PBS_O_WORKDIR
progressiveMauve --seed-family --seed-weight=11 --backbone-output=backbone_output --output=output.mauve seq1 Amphimedon_queenslandica_genomic_scaffolds.fa seq2 Mnemiopsis_leidyi_genomic_scaffolds.fa seq3 Monosiga_brevicollis_genomic_scaffolds.fa seq4 Nematostella_vectensis_genomic_scaffolds.fa seq5 Pleurobrachia_bachei_genomic_scaffolds.fa seq6 Trichoplax_adhaerens_genomic_scaffolds.fa

_The error I'm getting:_
########################### Execution Started #############################
JobId:1134043.paroo3
UserName:uqkkocot
GroupName:uq
ExecutionHost:b10a09
WorkingDir:/var/spool/PBS/mom_priv
###############################################################################
Exception FileNotOpened thrown from
Unknown()  in gnFileSource.cpp 67
Called by Unknown()
/var/spool/PBS/mom_priv/jobs/1134043.paroo3.SC: line 7: 15522 Floating point exceptionprogressiveMauve --seed-family --backbone-output=backbone_output --output=output.mauve seq1 Amphimedon_queenslandica_genomic_scaffolds.fa seq2 Mnemiopsis_leidyi_genomic_scaffolds.fa seq3 Monosiga_brevicollis_genomic_scaffolds.fa seq4 Nematostella_vectensis_genomic_scaffolds.fa seq5 Pleurobrachia_bachei_genomic_scaffolds.fa seq6 Trichoplax_adhaerens_genomic_scaffolds.fa ########################### Job Execution History #############################
JobId:1134043.paroo3
UserName:uqkkocot
GroupName:uq
JobName:Mauve
SessionId:15423
ResourcesRequested:mem=64gb,ncpus=1,place=free,walltime=300:00:00
ResourcesUsed:cpupercent=0,cput=00:00:00,mem=1472kb,ncpus=1,vmem=11508kb,walltime=00:00:03
QueueUsed:workq
AccountString:uq-Biology
ExitStatus:136
###############################################################################

Thank you very much!
Kevin Kocot
--
Kevin Kocot, Ph.D.
Postdoctoral Researcher
The University of Queensland
School of Biological Sciences
325 Goddard Building 8
St. Lucia, QLD 4072
Australia
Ph: +61 0402 488 430

--
Kevin Kocot, Ph.D.
Postdoctoral Researcher
The University of Queensland
School of Biological Sciences
325 Goddard Building 8
St. Lucia, QLD 4072
Australia
Ph: +61 0402 488 430

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