Hello !

 Am a new user for Mauve and I tried aligning two mammalian bat
genomes (rna.gbk files) but the error code 134 was returned and I am
not able to find out a solution for the same. I used the Progressive
Mauve and retained the default values for parameters and scoring. Do
suggest a solution. The console log is as follows.

OS name is: Linux arch: i386
trying path /home/mku/Downloads/mauve_2.3.1/progressiveMauve
Executing:
/home/mku/Downloads/mauve_2.3.1/progressiveMauve
--output=/home/mku/Pictures/mauve
--output-guide-tree=/home/mku/Pictures/mauve.guide_tree
--backbone-output=/home/mku/Pictures/mauve.backbone /media/SWETHA/My
Project!/Sequences/M.brandtii/M.brandtii rna.gbk /media/SWETHA/My
Project!/Sequences/P.alecto/P.alecto  rna.gbk
Storing raw sequence at /tmp/rawseq7653.000
Sequence loaded successfully.
/media/SWETHA/My Project!/Sequences/M.brandtii/M.brandtii rna.gbk
81248420 base pairs.
Storing raw sequence at /tmp/rawseq7653.001
Sequence loaded successfully.
/media/SWETHA/My Project!/Sequences/P.alecto/P.alecto  rna.gbk
57233395 base pairs.
Using weight 19 mers for initial seeds
Creating sorted mer list
Create time was: 77 seconds.
Creating sorted mer list
Create time was: 55 seconds.
0%..1%..2%..3%..4%..5%..6%..7%..8%..9%..10%..
11%..12%..13%..14%..15%..16%..17%..18%..19%..20%..
21%..22%..23%..24%..25%..26%..27%..28%..29%..30%..
31%..32%..33%..34%..35%..36%..37%..38%..39%..40%..
41%..42%..43%..44%..45%..46%..47%..48%..49%..50%..
51%..52%..53%..54%..55%..56%..57%..58%..59%..60%..
61%..62%..63%..64%..65%..66%..67%..68%..69%..70%..
71%..72%..73%..74%..75%..76%..77%..78%..79%..80%..
81%..82%..83%..84%..85%..86%..87%..88%..89%..90%..
91%..92%..93%..94%..95%..96%..97%..98%..99%..done.
using default bp penalty: 182316
using default bp estimate min score: 546948
Starting with 189877 multi-matches
Computing genome content distance matrix...


Genome conservation distance matrix:
0       0.786507
0.786507        0

Writing guide tree to /home/mku/Pictures/mauve.guide_tree
reading tree...
initializing alignment tree...
Constructing seed occurrence lists for repeat detection
Calculating pairwise breakpoint distances
Pair 0, 1 has 57809 initial LCBs
Using scaled bp penalty: 4000
Pair (0,1) has 7854 well-supported breakpoints
genome content distance matrix:
0       0.786507
0.786507        0

bp distance matrix:
0.9     0.9
0.9     0.9

Aligning...
Aligning node 1 to 2 via 0!
get ancestral matches
Performing Sum-of-pairs Greedy Breakpoint Elimination
construct LCB tracking matches
There are 172041 tracking matches
There are 344082 / 516123 components used
init tracking match LCB tracking
pairwise score tracking matches
get pairwise LCBs
there are 57731 pairwise LCBs
scaling bp penalty by conservation weight:
0.786507


scaling bp penalty by bp weight:
0.9

Greedy BPE
Scoring with scaled breakpoint penalty: 4000
1%..2%..3%..4%..5%..6%..7%..8%..9%..
10%..11%..12%..13%..14%..15%..16%..17%..18%..19%..
20%..21%..22%..23%..24%..25%..26%..27%..28%..29%..
30%..31%..32%..33%..34%..35%..36%..37%..38%..39%..
40%..41%..42%..43%..44%..45%..46%..47%..48%..49%..
50%..done
Arrived at 8076 intervals
Adding unaligned intervals
addUnalignedIntervals yields 24211 intervals
Merging unaligned intervals
Marbling gaps
Propagating descendant breakpoints
descendant 0(1) has 1 intervals
descendant 1(2) has 1 intervals
propagateDescendantBreakpoints yields 8076 intervals
Creating ancestral ordering
Previous anchoring score: -1.79769e+308, new anchor score: 8.83463e+08
Backing up alignment tree...
propagating ancestral breakpoints
recursive anchor search
Creating dmSML for seqI 0
Wrote raw sequence for seqI 0
Loaded sequence 023837698b.p.
MemTotal:        3966888 kB
Crap.  It's broke, return value 1
terminate called after throwing an instance of 'genome::gnException'
Exited with error code: 134

------------------------------------------------------------------------------
Dive into the World of Parallel Programming. The Go Parallel Website,
sponsored by Intel and developed in partnership with Slashdot Media, is your
hub for all things parallel software development, from weekly thought
leadership blogs to news, videos, case studies, tutorials and more. Take a
look and join the conversation now. http://goparallel.sourceforge.net/
_______________________________________________
Mauve-users mailing list
Mauve-users@lists.sourceforge.net
https://lists.sourceforge.net/lists/listinfo/mauve-users

Reply via email to