Hi Amarin, I realize this reply is coming a bit late but hopefully
better late than never. There was a nasty bug in 2.4.0 which caused
frequent crashes. It's fixed in a snapshot release that was made in late
February. I've now linked the snapshot directly from the download page
on the Mauve site:
http://darlinglab.org/mauve/download.html

Hope this helps,
-Aaron

On Thu, 2015-04-23 at 06:42 +1000, Amarin Cogburn wrote:
> Aaron,
> 
>  
> 
> I can’t seem to get the latest version of Mauve to run on my windows
> laptop or workstation.  I've tried alignments using two different
> bacterial genomes but the program exits with the same error code when
> writing the guide tree each time. Any input would be appreciated.
> Thanks. 
> 
>  
> 
> Running alignment.
> 
> Executing 
> 
>   win64\progressiveMauve
> 
>     --output=K12_TolC_Alignment
> 
>     --output-guide-tree=K12_TolC_Alignment.guide_tree
> 
>     --backbone-output=K12_TolC_Alignment.backbone
> 
>     C:\Users\xz3768\Desktop\K12_DH10Bgenome.fa
> 
>     C:\Users\xz3768\Desktop\K12_TOLC_contigs.fas
> 
>     C:\Users\xz3768\Desktop\K12_TOLC_parent_contigs.fas
> 
> Storing raw sequence at C:\Users\xz3768\AppData\Local\Temp\raw1401.tmp
> 
> Sequence loaded successfully.
> 
> C:\Users\xz3768\Desktop\K12_DH10Bgenome.fa 4686137 base pairs.
> 
> Storing raw sequence at C:\Users\xz3768\AppData\Local\Temp\raw1662.tmp
> 
> Sequence loaded successfully.
> 
> C:\Users\xz3768\Desktop\K12_TOLC_contigs.fas 4715635 base pairs.
> 
> Storing raw sequence at C:\Users\xz3768\AppData\Local\Temp\raw1808.tmp
> 
> Sequence loaded successfully.
> 
> C:\Users\xz3768\Desktop\K12_TOLC_parent_contigs.fas 4710259 base
> pairs.
> 
> Using weight 15 mers for initial seeds
> 
> Creating sorted mer list
> 
> Create time was: 2 seconds.
> 
> Creating sorted mer list
> 
> Create time was: 2 seconds.
> 
> Creating sorted mer list
> 
> Create time was: 2 seconds.
> 
> 0%..1%..2%..3%..4%..5%..6%..7%..8%..9%..10%..
> 
> 11%..12%..13%..14%..15%..16%..17%..18%..19%..20%..
> 
> 21%..22%..23%..24%..25%..26%..27%..28%..29%..30%..
> 
> 31%..32%..33%..34%..35%..36%..37%..38%..39%..40%..
> 
> 41%..42%..43%..44%..45%..46%..47%..48%..49%..50%..
> 
> 51%..52%..53%..54%..55%..56%..57%..58%..59%..60%..
> 
> 61%..62%..63%..64%..65%..66%..67%..68%..69%..70%..
> 
> 71%..72%..73%..74%..75%..76%..77%..78%..79%..80%..
> 
> 81%..82%..83%..84%..85%..86%..87%..88%..89%..90%..
> 
> 91%..92%..93%..94%..95%..96%..97%..98%..99%..done.
> 
> using default bp penalty: 155158
> 
> using default bp estimate min score: 465475
> 
> Starting with 1047 multi-matches
> 
> Computing genome content distance matrix...
> 
>  
> 
>  
> 
> Genome conservation distance matrix: 
> 
> 0              0.0771738            0.0795964
> 
> 0.0771738            0              0.0060033
> 
> 0.0795964            0.0060033            0
> 
>  
> 
> Writing guide tree to K12_TolC_Alignment.guide_tree
> 
> Exited with error code: -1073741819
> 
> 
> 

-- 
Aaron E. Darling, Ph.D.
Associate Professor, ithree institute
University of Technology Sydney
Australia

http://darlinglab.org
twitter: @koadman





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