Dear All,


I am using Mauve (progressive Mauve) to align whole genomes of two closely 
related genomes (grasscarp and zebrafish) and the objective is to determine the 
extent inter and intra-chromosomal rearrangements.



For this comparison, a seed weight of 19 and LCB weight of 2000 was used.  The 
resulting .XMFA alignment file was uploaded in the Mauve viewer. I am able to 
visualise the rearrangements between these two genomes.



However I couldn't see any representation for the "inter-chromosomal 
boundaries" i.e. a line that demarcates the neighbouring chromosomes in the 
reference genome. From the Mauve viewer, View--> Styles, the option for 
Chromosome/contig boundaries is turned on. Still, unable to see any lines that 
demarcate the chromosome boundaries (except the beginning and end of the whole 
genome). The sequences of all chromosomes are concatenated and the coordinates 
of the concatenated chromsomes are shown, without any marking demarcating the 
chromosomes.



I have attached a snapshot of the view (have turned off the LCB connecting 
lines for this snapshot). Can you kindly advise if there a way to visualise the 
boundary lines for each chromosome?. Also appreciate if you can comment on the 
suitability of the parameters (see weight and LCB weight) used by me for 
aligning these two genomes.



Thank you,
Kind regards,
Nisha


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