The following message is official and has been approved by the appropriate authorities to be posted to this list. ++++++++++++++ *RESEARCH ASSISTANT - Genome Biology *
Requisition # 019092 The Life Sciences Division of Berkeley Lab has an opportunity for a Research Assistant in the Genome Biology Department. Under the direct supervision of a Staff Scientist, this position will assist the Principal Investigator (PI) with investigating how changing the expression of components of mitotic spindles, nuclei and centrosomes affects the accuracy of mammalian cell division. The research involves cell and molecular biologic approaches as well as biochemical techniques. The recent determination of the genomic sequences of yeast, nematodes, fruit flies, mice, and humans is a watershed in biology. The Genome Biology Department is focused to take advantage of this wealth of new information. It is well known that DNA encodes the basic blue print of life, but it is not known how to interpret most of this information, particularly that from animals with large genomes. To address this issue, laboratories within the department are developing computational, biochemical, and genetic methods to decipher the complex sequence motif that control RNA transcription, DNA replication, and chromosome structure. http://www.lbl.gov/lifesciences/departments/genome_biology.html *Key Responsibilities:* *Prepare cells for immunofluorescent microscopy and assist with collection of digital images *Culture mammalian cells and perform RNAi transfections to silence protein expression *Perform immunoprecipitations and Western blotting *Prepare cell populations for cell cycle analysis *Purify antibodies *Express and affinity purify proteins from bacteria *Maintain accurate and detailed records of experiments and assist in preparation of materials for presentations and publications including Photoshop and Illustrator (or equivalent) *May assist with management of daily lab operations, including preparation of buffers, reagents, media and ordering supplies *Qualifications: * *College level course work in biochemistry, cell biology and/or molecular biology with specific experience with one or more of the following: cell culture, cell fixation for immunofluorescence immunoprecipitation, digital imaging, Western blotting, cell transfection, expression and purification of proteins in bacteria. *Familiarity with both Macintosh and PC computers for data analysis and record keeping *Ability to understand disparities, positive and negative results in routine experimental procedures in order to report and discuss with supervisor *Excellent organizational and communication skills with the ability to follow both written and verbal direction *Ability to work as team member to accomplish research goals. /Note: This position is represented by a union for collective bargaining purposes./ / / LBNL is located in the San Francisco Bay Area on a 200-acre site in the hills above the University of California's Berkeley campus and is managed by the University of California. A leader in science and engineering research for more than 70 years, LBNL is the oldest of the U.S. Department of Energy's National Laboratories. For more information about LBNL and its programs, visit www.lbl.gov. Apply now at http://jobs.lbl.gov/LBNLCareers/details.asp?jid=3D19092&p=3D1 to be immediately considered for this opportunity. Use Internet Explorer for best results. Attach your r=E9sum=E9 and cover letter as one document. Please reference "College Recruiting" and "UC Berkeley" as your source. LBNL is an Affirmative Action/Equal Opportunity Employer committed to a safe and diverse workforce. **************************************** Amy Pagsolingan, PHR - Senior Recruiter Lawrence Berkeley National Laboratory http://jobs.lbl.gov One Cyclotron Road, MS: 90-2121 Berkeley, CA 94720 Phone: 510-486-5277 Fax: 510-486-5066 [EMAIL PROTECTED] <mailto:[EMAIL PROTECTED]> ---------------------------------------------------------------- The above message does not necessarily represent the views or opinions of MCBcDNA, mcbUSA, the MCB Department, or UC Berkeley. TO UNSUBSCRIBE FROM THIS LIST, send an email to [EMAIL PROTECTED] with "unsubscribe mcb-infoline" in the message body. Please send questions or comments to [EMAIL PROTECTED] For a list of upcoming events and information about the student clubs: MCBcDNA http://mcb.berkeley.edu/groups/mcbcdna/ mcbUSA http://mcb.berkeley.edu/groups/mcbusa/
