http://www.pnas.org/cgi/content/abstract/0832464100v1

Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0832464100

Geology
Nanobacteria-like calcite single crystals at the
surface of the Tataouine meteorite 

Karim Benzerara *, Nicolas Menguy *, Fran�ois Guyot *, Christian Dominici , 
and Philippe Gillet  

*Laboratoire de Min�ralogie-Cristallographie, Unit� Mixte de Recherche 7590
Centre National de la Recherche Scientifique and Institut de Physique du Globe 
de Paris, 4 Place Jussieu, 75252 Paris
Cedex, France; Laboratoire CP2M Facult� des Sciences et Techniques de 
Saint Jer�me, Universit� d'Aix-Marseille, III,
F-13397 Marseille Cedex 20, France; and Laboratoire des Sciences de la Terre, 
Ecole Normale Sup�rieure Lyon, 46 All�e d'Italie, 69007 Lyon Cedex, France

Communicated by Russell J. Hemley, Carnegie Institution, Washington, DC, 
April 24, 2003 (received for review March 8, 2003)

Nanobacteria-like objects evidenced at the surface of the orthopyroxenes of the 
Tataouine meteorite in South Tunisia have been studied by scanning and 
transmission electron microscopies. A method of micromanipulation has been 
developed to ensure that exactly the same objects were studied by both methods. 
We have shown that the nanobacteria-like objects are spatially correlated with 
filaments of microorganisms that colonized the surface of the meteoritic 
pyroxene during its 70 years of residence in the aridic Tataouine soil. 
Depressions of a few micrometers in depth are observed in the pyroxene 
below the carbonates, indicating preferential dissolution of the pyroxene and 
calcite precipitation at these locations. The nanobacteria-like small rods 
that constitute calcium carbonate rosettes are well crystallized calcite 
single crystals surrounded by a thin amorphous layer of carbonate composition 
that smoothes the crystal edges and induces rounded shapes. Those morphologies 
are unusual for calcite single crystals observed in natural samples. A survey 
of recent literature suggests that the intervention of organic compounds 
derived from biological activity is likely in their formation.

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