Depending on your application, you may consider filter concentrators or
dialysis.
If you're using the columns to desalt or buffer exchange your sample,
you could use a centrifugation concentrator with a molecular weight
cutoff just below your protein (or whatever) of interest. Concentrate,
then dilute your sample with your new buffer... concentrate, then dilute
your sample with your new buffer.
Corning Spin-X Ultra Filtration Concentrators
http://www.fisher.co.uk/offers/index.php?pid=354
Alternatively, you could use dialysis membrane, and dialyze your sample
several times against your new buffer. Much cheaper.
Nick Theodorakis wrote:
On Feb 2, 5:26 am, WS <novalidaddr...@nurfuerspam.de> wrote:
Dear Experts,
is there any commercial (and preferably cheaper) alternative to PD-10
desalting columns?
(They are filled with Sephadex G-25, sample volume is up to 2.5ml,
elution volume is 3.5ml).
Buy the empty disposable columns and fill them yourself.
Alternatively, if you can work with low (100 ul) volumes, you can pour
them in 1cc syringes and do spin-column desalting.
Nick
--
Nick Theodorakis
nick_theodora...@hotmail.com
contact form:
http://theodorakis.net/contact.html
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Arne K Christensen
Postdoctoral Research Associate
University of Massachusetts, Amherst
USGS Conte Anadromous Fish Research Center
One Migratory Way, PO Box 796
Turners Falls, MA 01376
Email: ar...@bio.umass.edu
Phone: (413) 863-3827
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