Dear Bionetters,

 

Many times I observed that before gel purification the intensity of the DNA
band on the gel would be very high. When this intense band is sliced and
purified by gel extraction using the spin columns its intensity decreases.


 

Could any one suggest the procedure to increase the efficiency of the spin
columns? 

 

Thanking you in advance. 

 

Regards,

Adroit

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