Hello, I work with BACs a lot. I retrieve the BAC sequences from ensemble. I guess you know which genomic region is in that BAC, a particular gene etc.. So I search for that gene in ensemble, and then go to the "Region in detail", then you have to make BAC clones visible on the map. To do this, on the left side click "Configure this page", and then from the menu go to "external data", and here you have to activate "BAC map". Then you go back to the map, and the BAC clones in that region should be listed under the map, so you select your clone and you can see which genomic region it corresponds, then you can retrieve that genomic sequence.
Iraz Toprak Aydin EPFL SV ISREC, Station 19 Batiment SV, SV 2540 CH-1015 Lausanne Switzerland Tel: +41 21 693 07 36 e-mail: iraz.ay...@epfl.ch -----Original Message----- From: methods-boun...@oat.bio.indiana.edu [mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of laure d'astarac Sent: 11 February 2010 13:32 To: meth...@magpie.bio.indiana.edu Subject: BAC informations Hi, I am looking for map restriction and sequence details on a genomic mouse BAC backbone, namely: RP24-304O8 Nothing is available on the clone registry from NCBI and corresponding weblinks. If someone can help me to find these informations... Thanks for your help, Laure _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
