Hello there,

 

I have already done that by electroelution... I really don't know if it's 
exactly what you seek.

Here are some protocols that can help you:

http://www.protocol-online.org/prot/Molecular_Biology/DNA/DNA_Extraction___Purification/DNA_Extraction_from_Agarose_Gel/Electro-elution_of_DNA/index.html


Hope this helps,

 

Sincerely

 

Mariana Ferrarini.

 
> From: cogniz...@googlemail.com
> To: meth...@magpie.bio.indiana.edu
> Date: Tue, 16 Feb 2010 22:31:19 +0530
> CC: 
> Subject: Gel Extraction without columns?
> 
> Dear Bionetters,
> 
> 
> 
> Does anyone have a protocol or suggestion to purify the PCR products using
> the gel extraction method without the use of spin columns?
> 
> 
> 
> Your help is greatly appreciated.
> 
> 
> 
> Thanks,
> 
> Adroit
> 
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> Methods mailing list
> Methods@net.bio.net
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