hi everybody, Can anyone suggest me a protocol of native page.I have tried the standard protocol of removing the sds from SDS-page protocol but my proteins are unable to enter the resolving gel(pH8.8).Also i am not sure about the sample buffer. I have tried proteins of various pI values 4,6,8 but i only get a vertical streak. Can my proteins get precipitated in sample buffer of pH6.8 since my proteins are in Tris buffer pH8 from which i aliquot for preparing samples for native page.Any suggestions will be highly appreciated. Thanks in advance. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
