Hi Ketut, if you can't find any special procedure for your receptor in the literature, I'd try simple fractionation:
first, disrupt your cells in a buffer that does not contain detergents. This will remove the cytosol. Remainder should be able for recovery at 15.000g. Wash the pellet a few times with this first buffer. Then extract the pellet with a buffer containing 0.5 to 1% of nonionic detergent. This should solubilize most of the membrane proteins, except those from resistant structures like the lipid rafts. When you spin again, you will sediment the nuclei. HTH, Wo _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
