Am 11.03.2010, 21:33 Uhr, schrieb lautys <lau...@gmail.com>:
how about lyophilize low protein concentration sample and resuspend in
lower
volume of buffer/water? is that recommended for protein concentration?
you can do that, but you would also concentrate all the other stuf in your
sample. The Wessel & Flügge method, and also Bensadoun & Weinstein (DOC +
TCA) have the advantage that you remove some interfereing materials like
salts, detergent etc. Also, they are quicker than lyophilisation and
easier to handle with a large number of samples.
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