On Mar 20, 12:44 am, WS <novalidaddr...@nurfuerspam.de> wrote: > Hi Ed, > > my 2c: unique events during the first cycles, then amplified. Sort of > unusual "single copy detection"... > > Best regards, > > Wo
These are not dimers but rather non-specific amplification which is different. I occasionaly obtain such result when there is target cDNA in the sample but is too degraded. You can use standard techniques like DMSO, Ta change, MgCl2 to deal with it. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
