Hi Bern:

Here is a link to the swedish Proteinatlas that are validating commercial 
antibodies, but also producing their own (sold in the US by Sigma, the 
"Prestige antibodies"). I have used their APP antibody in the past. They have 
validated one antibody for Sestrin 2, and you find tissue sections etc. on line.

http://www.proteinatlas.org/
Just search for Sestrin.

Hope this helps.

Susanne




On Apr 8, 2010, at 7:59 AM, Bernard Martin wrote:

> Hi all,
> 
> I asked this a couple weeks ago, but thought I'd give it one more try- 
> 
> has anyone had any experience with Sestrin 2 for IHC?  
> 
> I can't find anything in the literature, and thought maybe some has tried it. 
>  There are ones available, but they all use the same picture, and I am 
> concerned perhaps it doesn't work well.  Funds are limited so we would like 
> to get one that someone has had some success with.  Thanks for any info you 
> could provide!
> 
> Bern
> 
> 
> 
> 
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